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Project Partners

Prof. Dr. Martin Steup

Institute of Biochemistry and Biology / Plant Physiology
University of Potsdam
Karl-Liebknecht-Str. 24-25, Building 20
14476 Potsdam-Golm
Tel 0331-977-2650
Fax 0331-977-2512
msteup@rz.uni-potsdam.de

Core Competency:

Regulation of intracellular carbon fluxes and the starch- and photosynthesis-related interaction between chloroplasts and cytosol. Methods include carbohydrate and protein analyses by mass spectrometry (including covalent modifications), protein-protein and protein-carbohydrate interactions and 14C in vivo pulse or pulse chase labelling of cells or plants.

Contribution to Work Programme:

WP1.2: Analysis of starch-related enzymes, protein-protein interactions, glycans and covalent modifications of glycans in Chlamydomonas.
WP 2: In vivo pulse and pulse chase labelling studies on Chlamydomonas and tomato to analyse carbon fluxes.
WP 3-4: Detailed analysis of the structure of starch-related glycans in Chlamydomonas and tomato during light and dark period; identification of interacting proteins and enzymes. The major contribution of this partner will be to WP3.

GoFORSYS Publications of the Group:

Fettke, J., M. Hejazi, et al. (2009) Eukaryotic starch degradation: integration of plastidial and cytosolic pathways. Journal of Experimental Botany.

Hejazi, M., J. Fettke, et al. (2009) The two plastidial starch-related dikinases sequentially phosphorylate glucosyl residues at the surface of both A- and B-type allomorph of crystallized maltodextrins but the mode of action differs. Plant Physiology.