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Project Partners

Prof. Dr. Ralph Bock, Director

Department 3 at the Max Planck Institute of Molecular Plant Physiology
Am Muehlenberg 1
14474 Potsdam-Golm
Tel 0331-567-8700
Fax 0331-567-8701
rbock@mpimp-golm.mpg.de

Core Competency:

Generation and characterization of photosynthetic mutants. Methods: nuclear and chloroplast transformation, spectroscopic quantitation of all components of the electron transport chain, Chl fluorescence, gas exchange, analysis of gene expression at all levels; experience with Chlamydomonas (member of the DFG-funded consortium FOR504).

Contribution to Work Programme: 

WP3.1 and 3.2: Chlamydomonas growth and transformation;

WP3.3 and 4: Analysis of the light reactions in Chlamydomonas and the two higher plants (also WP2.1: quantitation of all components of the electron transport chain in vivo: difference absorption spectroscopy, gas exchange, chlorophyll fluorescence); Analysis of gene expression: determination of transcriptional and translational responses in the three major classes of non-polyadenylated RNAs (chloroplast and mitochondrial transcripts, microRNAs): (i) steady-state transcript levels, (ii) translational activities (analyzing polysomal RNAs on microarrays). Chlamydomonas non-coding RNAs will be identified by an experimental RNomics approach. The major contribution of this partner will be to WP3.

GoFORSYS Publications of the Group:

Schöttler, M. A. and Bock, R. (2007). Plastids-nuclear cooperation in photosystem I assembly in photosynthetic eukaryotes. Progress in Botany 69, 89-115.

May, P., S. Wienkoop, et al. (2008). "Metabolomics- and proteomics-assisted genome annotation and analysis of the draft metabolic network of Chlamydomonas reinhardtii." Genetics 179(1): 157-166.

Nguyen, A. V., S. R. Thomas-Hall, et al. (2008). "Transcriptome for photobiological hydrogen production induced by sulfur deprivation in the green alga Chlamydomonas reinhardtii." Eukaryotic cell 7(11): 1965-79.

Shao, N. and Bock, R. (2008). A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii. Current Genetics 53(6):381-388.

Lau, S., N. Shao, et al. (2009). "Auxin signaling in algal lineages: fact or myth?" Trends in Plant Science 14(4): 182-8.

Rupprecht, J. (2009). "From Systems Biology to Fuel – Chlamydomonas reinhardtii as a model for a systems biology approach to improve biohydrogen production." Journal of Biotechnology.

Neupert, J., D. Karcher, et al. (2009). "Generation of Chlamydomonas strains that efficiently express nuclear transgenes." Plant Journal 57(6): 1140-50.