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PD Dr. Michael Schroda

University of Potsdam, Institute of Biochemistry and Biology/Genetics c/o Max Planck Institute of Molecular Plant Physiology
Am Muehlenberg 1
14474 Potsdam-Golm
Tel 0331-567-8109
Fax 0331-567-8136
schroda@mpimp-golm.mpg.de

Core Competency:

Protein identification and quantification based on mass spectrometry, stable isotope labelling and 15NH4Cl labelling. Metabolite profiling using GC-TOF-MS. Chromatin immunoprecipitation. Analysis of protein-protein interactions. Transgene expression and RNAi approaches in Chlamydomonas. Gene identification. Long-standing experience with Chlamydomonas (member of the DFG-funded consortium FOR504, co-author of the current Chlamydomonas Sourcebook).

Contribution to Work Programme:

Large scale analysis of changes in metabolite and protein levels following well-defined changes in growth conditions (WP2 and 3). Genetic manipulations of Chlamydomonas for refinement and testing of models developed in WP1.4. Target sites for key transcription factors on a genome-wide scale will be identified by ChIP-seq.

GoFORSYS Publications of the Group: (incl. thosey former Group Leader Wolfram Weckwerth)

Hummel, J., M. Niemann, et al. (2007). "ProMEX: a mass spectral reference database for proteins and protein phosphorylation sites." Bmc Bioinformatics 8: -.

Merchant, S. S., S. E. Prochnik, et al. (2007). "The Chlamydomonas genome reveals the evolution of key animal and plant functions." Science 318(5848): 245-251.

Muller-Linow, M., Weckwerth, W., and Hutt, M. T. (2007) Consistency analysis of metabolic correlation networks. Bmc Systems Biology 1, -.

Reumann, S., L. Babujee, et al. (2007). "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms." Plant Cell 19(10): 3170-3193.

Heazlewood, J. L., P. Durek, et al. (2008). "PhosPhAt: a database of phosphorylation sites in Arabidopsis thaliana and a plant-specific phosphorylation site predictor." Nucleic Acids Research 36: D1015-D1021

Heide, H., A. Nordhues, et al. (2008). "Application of quantitative mass spectrometry combined with immunoprecipitation, knock-down and cross- linking (QUICK-X) to Chlamydomonas reveals the interaction of VIPP1 with chloroplast SP90C." Proteomics.

Hoehenwarter, W., J. T. van Dongen, et al. (2008). "A rapid approach for phenotype-screening and database independent detection of cSNP/protein polymorphism using mass accuracy precursor alignment." Proteomics 8(20): 4214-4225.

Lehmann, U., S. Wienkoop, et al. (2008). "If the antibody fails - a mass Western approach." Plant Journal 55(6): 1039-1046.

Weckwerth, W. (2008). Metabolomics und Datenintegration in der Systembiologie, BMBF.

Weckwerth, W. (2008). "Integration of metabolomics and proteomics in molecular plant physiology - coping with the complexity by data-dimensionality reduction." Physiologia Plantarum 132(2): 176-189.

Weckwerth, W. (2008). Metabolomics und Datenintegration in der Systembiologie, BMBF.

Wienkoop, S., K. Morgenthal, et al. (2008). "Integration of metabolomic and proteomic phenotypes." Molecular & Cellular Proteomics 7(9): 1725-1736.

Kempa, S., J. Hummel, et al. (2009). "An automated GCxGC-TOF-MS protocol for batch-wise extraction and alignment of mass isotopomer matrixes from differential C-13-labelling experiments: a case study for photoautotrophic-mixotrophic grown Chlamydomonas reinhardtii cells." Journal of Basic Microbiology 49(1): 82-91.

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