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Project Partners

Prof. Dr. Mark Stitt, Director

Department 2, at the Max Planck Institute of Molecular Plant Physiology
Am Muehlenberg 1
14474 Potsdam-Golm
Tel 0331-567-8100
Fax 0331-567-8101
mstitt@mpimp-golm.mpg.de

Core Competency:

The regulation of metabolism and growth by carbon and nutrients. Methods include visualisation tools, transcript profiling (including robotised RT-PCR), robotised enzyme activity profiling and metabolite profiling (including targeted LC-MS/MS analyses).

Contribution to Work Programme:

WP1: the Mapman ontology and data visualisation tool will be adapted to Chlamydomonas to allow parallel analysis / display of data from this model alga, Arabidopsis and tomato, and links from all species into Arabidopsis resources like CSBDB.

WP2: methods for robotised qRT-PCR, enzyme profiling and LC-MS/MS will be adapted to Chlamydomonas and methods developed for quantify protein synthesis.

WP3-4: contributions to multilevel phenotyping of Chlamydomonas, Arabidopsis and tomato, including >45 enzyme activities, phosphorylated intermediates, sugars, starch and protein, and measurement of translation and protein turnover. The major contribution of this partner will be to WP3.

GoFORSYS Publications of the Group:

Usadel, B., O. Bläsing, et al. ( ) Temporal Responses of Metabolites and Global Transcript Levels to Progressive Exhaustion of Carbohydrates in Arabidopsis Rosettes. Plant Physiology, in press.

May, P., S. Wienkoop, et al. (2008). "Metabolomics- and proteomics-assisted genome annotation and analysis of the draft metabolic network of Chlamydomonas reinhardtii." Genetics 179(1): 157-166.

Gibon, Y., M.-T. Pyl, et al. (2009) Adjustment of growth, starch turnover, protein content and central metabolism to a decrease of the carbon supply when Arabidopsis is grown in very short photoperiods. Plant Cell Environment, in press.

Arrivault S., M. Günter, et al. (2009) Measurement of Calvin cycle and other metabolic intermediates in Arabidopsis leaves at different carbon dioxide concentrations using reverse-phase liquid chromatography linked to tandem mass spectrometry. Plant Journal, in press.

Hannemann, J., H. Poorter, et al. (2009). "Xeml Lab: a tool that supports the design of experiments at a graphical interface and generates computer-readable meta-data files, which capture information about genotypes, growth conditions, environmental perturbations and sampling strateg." Plant, Cell & Environment.

Sulpice, R., E.-Th. Pyl, et al. (2009). Starch as a major integrator in the regulation of plant growth. PNAS in press